Methods for preparing oxazolidinones and compositions containing them

ABSTRACT

Methods of preparing a class of oxazolidinones useful to impede bacterial growth are disclosed.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation of U.S. application Ser. No.12/577,089, filed Oct. 9, 2009, which claims priority to U.S.Provisional Application No. 61/104,469, filed Oct. 10, 2008.

BACKGROUND OF THE INVENTION

Oxazolidinones as a chemical class find widespread use as pharmaceuticalagents for the therapy and prophylaxis of such medical conditions asbacterial infections and atherosclerosis. The utility of these compoundshas spurred efforts to find efficient routes to synthesize them, such asthe copper-catalyzed cross coupling disclosed in US 20070049759. US20070155798, which is hereby incorporated by reference in its entirety,recently disclosed potently anti-bacterial oxazolidinones that featuresubstituted pyridinyl phenyl moieties. These moieties were initiallyincorporated by synthetic routes involving tin-based couplings, whichbecause of the toxicity of any residual tin compounds is not desirablefor pharmaceutical use. Accordingly, a need exists for synthetic routesto substituted (pyridinyl)phenyl oxazolidinones that does not involveuse of tin reagents.

FIELD OF THE INVENTION

Novel methods are useful in the preparation of oxazolidinone-containingcompounds.

SUMMARY OF THE INVENTION

A method of synthesizing a compound of the structure

wherein

R is H,

R¹a and R¹b are independently selected from H and F, provided that atleast one of R¹a and R¹b is F,

Het is an optionally-substituted five- or six-membered heterocyclecomprising at least one N, O, or S atom,

comprises treating a compound having the structure

wherein R² is selected from the group consisting of optionallysubstituted benzyl and optionally substituted C₁-C₆ alkyl, with a strongbase or an organolitihium salt and then addition of glycidyl butyrate tothe resulting anion under conditions to make

In some aspects, the treating step is performed in the presence of afacilitating compound, such as1,3-dimethyl-3,4,5,6-tetrahydro-2(1H)-pyrimidinone.

In some embodiments, the method includes an additional step comprisingreacting

with POCl₃, POCl(OBn)₂, or P(N-iPr₂)(O-tBu)₂ under conditions form

wherein R′ is PO(OH)₂.

The method may also comprise treating the compound of the structure

where R′ is PO(OH)₂ with a base under conditions to form the compound ofthe structure

wherein R″ is a pharmaceutically acceptable salt of PO(OH)₂. In someaspects, the base is a sodium-containing base. In some aspects, R″ isPO₃Na₂

A separate method of making an intermediate, or an additional stepbefore the steps above, comprises coupling a first intermediate of thestructure

wherein X is a leaving group such as selected from the group consistingof Cl, Br, I, and trifluoromethanesulfonate, with a second intermediateof the structure

wherein Y is selected from the group consisting of ZnCl, BF₃, and BR³R⁴,wherein R³ and R⁴ are independently selected from the group consistingof OH and optionally-substituted C₁-C₆ mono and dihydric alcohols, andwherein R³ and R⁴ together may form a ring, under conditions to producethe compound of the structure

In some aspects, the coupling is carried out in the presence of apalladium complex such as phosphine ligand bound to palladium, forexample, dichlorobis(triphenyl-phosphine)palladium(II),tetrakis(triphenylphosphine)palladium(0), or Pd₂(dba)₃

A separate method of making an intermediate, or an additional stepbefore the coupling step above, comprises

-   -   a) treating an aryl halide of structure 5a

-   -   wherein X¹ is leaving group, with a strong base such as n-butyl        lithium and then reacting a resulting anion with a trialkylboric        acid ester under conditions to form

or

-   -   b) treating the aryl halide of structure 5a with a palladium        catalyst such as PdCl₂(dppf)₂ and a dipinacolate ester of        diboronic acid under conditions to form

In some embodiments, Y is selected from the group consisting of B(OH)₂,BF₃, and

In some embodiments, Het is selected from the group consisting ofoptionally-substituted pyrrole, furan, piperazine, piperidine,imidazole, 1,2,4-triazol, 1,2,3-triazol, tetrazole, pyrazole,pyrrolidine, oxazole, isoxazole, oxadiazole, pyridin, pyrimidine,thiazole or pyrazine, such as an optionally-substituted tetrazolylgroup, for example 2-methyl-tetrazol-5-yl.

In some embodiments, the method further comprises treating the compoundof the structure

with a glycidyl ester such as glycidyl butyrate. In some aspects theglycidyl ester has R stereochemistry, such as R-(−)-glycidyl butyrate.This treating step may be carried out in the presence of lithiumhexamethyldisilazide.

Compounds made from the processes described herein include

In some embodiments, a compound of the formula has the structure:

wherein;

R¹a and R¹b are independently selected from H and F, provided that atleast one of R¹a and R¹b is F,

R² is selected from the group consisting of optionally substitutedbenzyl and optionally substituted C₁-C₆ alkyl, and

Het is an optionally-substituted five- or six-membered heterocyclecomprising at least one N, O, or S atom.

In some embodiments, a compound of the formula has the followingstructure:

wherein

R¹a and R¹b are independently selected from H and F, provided that atleast one of R¹a and R¹b is F,

R² is selected from the group consisting of optionally substitutedbenzyl and optionally substituted C₁-C₆ alkyl, and

Y is selected from the group consisting of ZnCl, BF₃, and BR³R⁴, whereinR³ and R⁴ are independently selected from the group consisting of OH andoptionally-substituted C₁-C₆ mono and dihydric alcohols, and wherein R³and R⁴ together may form a ring.

In some aspects, a composition comprises the compound herein such asprepared in accordance with the processes herein and a dimer having thefollowing structure or a pharmaceutically acceptable salt of the dimer

-   -   wherein R¹a and R¹b are independently selected from H and F,        provided that at least one of R¹a and R¹b is F,    -   Het is an optionally-substituted five- or six-membered        heterocycle comprising at least one N, O, or S atom.

In some aspects, R¹a is F and R¹b is H and Het is2-methyl-tetrazol-5-yl.

In further embodiments a composition comprises the compound herein suchas prepared in accordance with the processes above, wherein thecomposition lacks tin impurities.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT

Methods are provided for synthesizing substituted(pyridinyl)phenyl-oxazolidinones

wherein Het is an optionally-substituted five- or six-memberedheterocycle comprising at least one N, O, or S atom such asoptionally-substituted tetrazolyl, oxazolyl, triazolyl, oxadiazolyl,thiazolyl, and isoxazolyl moieties. In some aspects, Het is anoptionally-substituted tetrazolyl such as 2-methyl-tetrazol-5-yl.

R¹a and R¹b are independently selected from H and F, provided that atleast one is F, and

R is selected from H, PO(OH)₂, and pharmaceutically acceptable salts ofPO(OH)₂.

Unless otherwise specified, technical terms here take their usualmeanings, specifically those specified in the McGraw-Hill Dictionary ofScientific and Technical Terms, 6^(th) edition.

In some embodiments, methods include synthesizing substitutedN-(pyridinyl)aryloxazolidinones by the following route ([0023] Scheme 1)

In Scheme 1, a first intermediate (4) is coupled in Rxn 1 with a secondintermediate (6) to afford a coupling product (7), which in Rxn 2 isthen treated with a glycidyl ester to afford compound (1).

In Scheme 2, intermediate 6 may be formed by treatment of intermediate5a with 2 equivalents of a strong base such as a C₁-C₆ alkyl lithium forexample n-butyl lithium or t-butyl lithium followed by the addition ofthe appropriate electrophile such as ZnCl₂ or B(OR)₃ i.e., C₁-C₆trialkoxyboronate such as triisopropyl boronate. Aqueous workup of theresulting reaction mixture where the electrophile is a trialkoxyborateester yields the boronic acid 6a. If the dianion of 5a is treated with acyclic boronate ester then the cyclic boronic acid ester 6b can beisolated. Further, if the electrophile is ZnCl2 then the zinc reagent 6ccan be isolated. Alternatively, the boronic acids may be prepared by theMiyaura boration procedure (Miyaura Top. Curr. Chem. 2002, 219, 11-59).In this reaction, a diester of diboronic acid such as dipinacolate esterof diboronic acid is coupled to an arylhalide (5a) using a palladiumcatalyst. The resulting boronic acid ester 6b can be hydrolyzed withaqueous acid to the boronic acid 6a. Further, the trifluoroboratederivative 6d can be formed from the boronic acid 6a by treatment withKF and/or KHF₂.

In the above schemes, X is a leaving group. In some embodiments, X isselected from Cl, Br, I, and trifluoromethanesulfonate.

X¹ is a leaving group. In some embodiments, X¹ is a halogen such as Cl,Br, or I.

Het is an optionally-substituted five- or six-membered heterocyclecomprising at least one N, O, or S atom, includingoptionally-substituted pyrrole, furan, piperazine, piperidine,imidazole, 1,2,4-triazol, 1,2,3-triazol, tetrazole, pyrazole,pyrrolidine, oxazole, isoxazole, oxadiazole, pyridin, pyrimidine,thiazole or pyrazine. In some aspects, Het is optionally-substitutedtetrazolyl or 2-methyl-tetrazol-5-yl. In some embodiments, Het isunsubstituted or has 1 or 2 substituents.

R¹a and R¹b are independently selected from H and F, provided that atleast one is F;

Y is selected from ZnCl, BF₃, and BR³R⁴, wherein R³ and R⁴ areindependently selected from OH and optionally-substituted C₁-C₆ mono anddihydric alcohols, and wherein R³ and R⁴ together may form a ring. Insome embodiments, Y is B(OH)₂ or pinacolatoborate, namely,

such as B(OH)₂. C₁-C₆ mono and dihydric alcohols may be optionallysubstituted with C₁-C₄ alkyl. A Negishi reaction may be performed toform compounds wherein Y is ZnCl (Negishi: Chem. Ind. 1988, 33,381-407).

In some embodiments, Het may be unsubstituted or optionally substitutedwith one or more substituents, for example, independently selected fromthe group consisting of halogen, hydroxy, amino, C₁₋₄ alkylamino,di(C₁₋₄ alkyl)amino, cyano, nitro, C₁₋₄ alkyl, C₁₋₄ alkoxy, C₁₋₄ acyl,C₁₋₄ thioalkyl, C₁₋₄ thiooxoalkyl, halogen substituted C₁₋₄ alkyl andhalogen substituted C₁₋₄ alkoxy.

Also in Scheme 1, R² is optionally substituted benzyl or optionallysubstituted C₁-C₆ alkyl. In some embodiments, benzyl and C₁-C₆ alkyl areunsubstituted or independently optionally substituted with halogen oralkoxy such as C₁-C₄ alkyloxy. In some embodiments, R² is benzyl and[0071] R is H.

Suitable catalysts for cross-coupling reaction Rxn 1 are palladiumcomplexes, for example palladium phosphine complexesordichlorobis(triphenylphosphine)-palladium(II),tetrakis(triphenylphosphine)palladium(0), and that prepared in situ fromPd₂(dba)₃ (dba=benzylideneacetone) in the presence of PCy₃. Theproportion of Pd complex to substrates to be coupled is not critical,but approximately 1 mole % (relative to either 4 or 6) has been found tobe useful.

Cyclization to produce the oxazolidinone ring is effected in Rxn 2 bytreating 7 with a strong base, such as lithium hexamethyldisilazide oran organolithium salt, such as n-butyl lithium, in the presence of1,3-dimethyl-3,4,5,6-tetrahydro-2(1H)-pyrimidinone (DMPU), followed by aglycidyl ester such as an R-(−)-glycidyl ester, for example, butyrate,to afford compound 1 (R═H). One embodiment uses lithiumhexamethyldisilazide as the base, and THF as the solvent, with DMPUpresent to facilitate the reaction, at a temperature between about 0° C.and about 30° C., and at a stoichiometry of 7 to glycidyl ester of about1:1 on a molar basis.

If desired, compound 1 (R═H) can further be converted to the dihydrogenphosphate, for example, by treatment with POCl₃, according to well-knownmethods. For example, compound 1 (R═H) can be treated with POCl₃followed by an aqueous quench or in a two step process using a protectedform of phosphorous oxychloride such as: POCl(OBn)₂ where the first stepprepares the phosphate triester and the second step removes theprotecting group (for example H₂/Pd—C to remove the benzyl esters).Alternatively, the 5-hydroxymethyl-oxazolidinone can be treated withP(N-iPr₂)(O-tBu)₂ followed by oxidation with an oxidizing reagent suchas mCPBA followed by treatment with base or aqueous acid to remove thetert-butyl esters).

The resulting dihydrogen phosphate compound 1 (R═PO(OH)₂) can further beconverted to a pharmaceutically acceptable salt such as the disodiumsalt of compound 1 (R═PO(O)₂ 2Na) by reaction with NaOMe or othersuitable sodium-containing base.

Those skilled in the art of medicinal chemistry will appreciate that theterm “pharmaceutically acceptable salt” refers to salts formed with suchbiologically compatible cations and/or anions, as appropriate. Suchcations include those of metallic elements, such as sodium, lithium,potassium, magnesium, aluminum, calcium, zinc, and quaternary cations oforganic nitrogenous bases, such as N,N′-dibenzylethylenediamine,chloroprocaine, choline, diethanolamine, ethylenediamine,N-methylglucamine and procaine-salts. Such anions include those ofinorganic acids, such as hydrochloric, hydrobromic, sulfuric,phosphoric, nitric, perchloric, fumaric, acetic, propionic, succinic,glycolic, formic, lactic, maleic, tartaric, citric, palmoic, malonic,hydroxymaleic, phenylacetic, glutamic, benzoic, salicylic, fumaric,toluenesulfonic, methanesulfonic, naphthalene-2-sulfonic,benzenesulfonic hydroxynaphthoic, hydroiodic, malic, steroic, tannic andsimilar acids.

Oxazolidinones prepared by the methods herein differ from theoxazolidinones that have been prepared in accordance with the US20070155798 method. Oxazolidinones made in accordance to the processdescribed herein do not contain tin impurities as no tin-containingreactants are used. In addition, in some embodiments, a dimer impurityhas been observed, for example, in batches in which phosphorusoxychloride (POCl₃) was used to convert hydroxyl to the dihydrogenphosphate. Specifically, a molecule of TR-701 reacts with a molecule ofphosphate ester containing at least one P—Cl bond to form the dimer,such as having the following structure.

The impurity is present in some detectable quantity and is present inless than about 10% by weight of the composition, and in some cases lessthan about 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2% or 1%, such as less than 0.1%or 0.05%. Thus, in some embodiments, compositions comprise anoxazolidinone as prepared in accordance with the process herein and adimer. In some embodiments compositions comprise an oxazolidinonelacking any tin impurities.

Oxazolidinones prepared by the methods herein are useful as medicaments,and particularly for impeding the growth of bacteria, as is disclosed indetail in US 20070155798, which has been incorporated by reference inits entirety.

The terms “approximately”, “about”, and “substantially” as used hereinrepresent an amount close to the stated amount that still performs thedesired function or achieves the desired result. For example, the terms“approximately”, “about” and “substantially” may refer to an amount thatis within less than 10% of, within less than 5% of, within less than 1%of, within less than 0.1% of, and within less than 0.01% of the statedamount.

EXAMPLES

The practice of the inventive method is illustrated by the followingnon-limiting example.

Experimental and Analytical Data

Reagents were purchased from commercial sources and were used asreceived. Proton nuclear magnetic resonance spectra were obtained on aBruker AVANCE 300 spectrometer at 300 MHz or an AVANCE 500 spectrometerat 500 MHz with tetramethylsilane used as an internal reference. Carbonnuclear magnetic resonance spectra were obtained on a Bruker AVANCE 500spectrometer at 125 MHz with the solvent peak used as the reference.Phosphorus nuclear magnetic resonance spectra were obtained on a BrukerAVANCE 500 spectrometer at 202 MHz with phosphoric acid used as thereference. Fluorine nuclear magnetic resonance spectra were obtained ona Bruker AVANCE 300 spectrometer at 282 MHz. Mass spectra were obtainedon a Finnigan AQA spectrometer with electrospray ionization. Thin-layerchromatography (TLC) was performed using Whatman No. 4500-101 (DiamondNo. MK6F silica gel 60 Å) plates. Visualization of TLC plates wasperformed using UV light (254 nm) or potassium permanganate stain. HPLCanalyses were obtained on a Varian Prostar HPLC equipped with a WatersSunFire C18 column (150×4.60 mm, 3.5 μm) or Waters XBridge C18 column(75 mm×4.6 mm×2.5 μm) using the methods below with the detector at thespecified wavelength.

Method A (Waters SunFire C18 Column)

Time (min) Flow (mL/min) % A % B 0.0 1.0 98.0 2.0 15.0 1.0 5.0 95.0 25.01.0 5.0 95.0 27.0 1.0 98.0 2.0 30.0 1.0 98.0 2.0 A = water with 0.05%v/v trifluoroacetic acid B = acetonitrile with 0.05% v/v trifluoroaceticacid Wavelength = 254 nm

Method B (Waters XBridge C18 Column)

1. Time 2. Flow (min) (mL/min) % A %B 0.0 1.0 98.0 2.0 15.0 1.0 5.0 95.025.0 1.0 5.0 95.0 27.0 1.0 98.0 2.0 30.0 1.0 98.0 2.0 A = 87% 25 mMammonium bicarbonate solution in water/13% acetonitrile B = acetonitrileWavelength = 254 nm

Method C (Waters SunFire C18 Column)

3. Time 4. Flow (min) (mL/min) % A %B 0.0 1.0 98.0 2.0 15.0 1.0 5.0 95.025.0 1.0 5.0 95.0 27.0 1.0 98.0 2.0 30.0 1.0 98.0 2.0 A = water with0.05% v/v trifluoroacetic acid B = acetonitrile with 0.05% v/vtrifluoroacetic acid Wavelength = 240 nm

Example 1 Preparation of 5-Bromo-2-(2H-tetrazol-5-yl)pyridine, 3

To a 22-L, three-neck, round-bottom flask equipped with an overheadstirrer, nitrogen inlet/outlet, thermocouple and heating mantle wascharged 5-bromo-2-cyanopyridine (799 g, 4.37 mol, 1 weight),N,N-dimethylformamide (6.4 L, 8 volumes), ammonium chloride (350.3 g,6.55 mol, 1.5 equivalents), and sodium azide (425.7 g, 6.55 mol, 1.5equivalents) while stirring. The internal reactor temperature set-pointwas adjusted to 85° C. (Target temperature is 90° C.). The temperatureset-point was reached after 45 minutes, and the reaction continued toself-heat to 94° C. over 40 minutes. The reaction was judged to becomplete after 1 hour by HPLC analysis by complete consumption of thestarting material with an assay of 76.7% (AUC) of the tetrazole ammoniumsalt. The mixture was cooled and filtered at room temperature. Thereactor and wet cake were washed with 2-propanol (3.2 L, 4 volumes) anddried under high vacuum at ambient temperature to afford the tetrazoleammonium salt as an off-white solid (847.9 g, 80% yield, 89.9% AUC). Adifferential scanning calorimetry experiment was conducted on thetetrazole ammonium salt to understand its thermal stability. The saltmelted at approximately 228° C. and had an energetic decomposition atapproximately 270° C.

Example 2 Preparation of 5-Bromo-2-(2-methyl-2H-tetrazol-5-yl)pyridine,4 (X═Br)

To a 22-L, four-neck, round-bottom flask equipped with an overheadstirrer, nitrogen inlet/outlet, and thermocouple placed in an ice/brinebath was charged the tetrazole ammonium salt (835.0 g, 3.44 mol, 1weight), tetrahydrofuran (7.5 L, 9 volumes), N,N-dimethylformamide (2.5L, 3 volumes) and sodium hydroxide powder (343.5 g, 8.59 mol, 2.5equivalents) while stirring. The internal reactor temperature wasallowed to reach 12° C., whereupon iodomethane (1.22 kg, 8.59 mol, 2.5equivalents) was added dropwise over 50 minutes, maintaining thereaction temperature below 20° C. After 20 minutes addition time, due toa rapid increase in temperature, the addition was paused and thereaction continued to self-heat from 15-20° C. over ten minutes. Theremainder of the addition was completed at constant temperature (18°C.). Upon completion of the addition, the ice/brine bath was removed andthe reactor was equipped with a water condenser and a heating mantle.The internal reactor temperature was adjusted to 40° C., however thereaction continued to self-heat to 48° C. The reaction was judged to becomplete after 6 hours by HPLC analysis by complete consumption of thestarting material. The reaction mixture was cooled to room temperatureovernight for convenience. The THF was removed by distillation, andwater (8.35 L, 10 volumes) was charged to the reactor. The slurry wasstirred for 30 minutes and filtered by vacuum filtration and the reactorand filter cake were washed with water (4.2 L, 5 volumes) to affordcrude 4/N1 isomer mixture as a peach colored solid (500.7 g, 61% yield,3.85: 1 4: N1).

The solids (500.7 g) were dissolved in CH₂Cl₂ (2.5 L, 5 volumes) towhich 6 N aqueous HCl (7.5 L, 15 volumes) was added. The biphasicmixture was stirred and the layers were separated. At this point, thedesired product is in the aqueous HCl layer. The CH₂Cl₂ layer was washedwith 6 N aqueous HCl (4.5 L, 3×3 volumes) until <5% AUC 4 was present byHPLC analysis. The combined 6 N HCl extracts were transferred to areactor and the pH was adjusted to 10.6 with 50% aqueous NaOH (˜3.2 L)while maintaining the internal temperature below 40° C. The solids wereisolated by vacuum filtration and the reactor and filter cake wererinsed with water (1 L, 2 volumes) to afford crude 4 as a yellow/orangesolid (322.4 g, 64% recovery, 39% yield, 93.5% AUC 4, 4.1% AUC N-1isomer) as confirmed by HPLC and ¹H NMR analyses.

The crude 4 was further purified by an isopropyl acetate (IPAc) reslurry(1.61 L, 5 volumes) at 50° C. for 1 hour. Upon cooling to roomtemperature, the solids were filtered and the reactor and filter cakewere washed with additional IPAc (500 mL, 1.6 volumes) to affordpurified 4 as a off-white/yellow solid (275.5 g, 85% recovery, 33%yield, 98.2% AUC) as confirmed by HPLC and ¹H NMR analyses. DSC analysisof 4 showed a decomposition exotherm at approximately 245° C.

Example 3 Preparation of benzyl (4-bromo-3-fluorophenyl)carbamate, 5

To a 12-L, three-neck, round-bottom flask equipped with an overheadstirrer, nitrogen inlet/outlet, addition funnel and thermocouple wascharged 4-bromo-3-fluoroaniline (800.0 g, 4.21 mol, Matrix lot #Q13H),THF (6.4 L, 8 vol), and solid sodium bicarbonate (530.5 g, 6.32 mol, 1.5eq). The addition funnel was charged with benzyl chloroformate (861.9 g,5.05 mol, 1.2 eq), which was added dropwise to the reactor over 70minutes. The temperature of the reaction was maintained below 20° C.with an ice water bath. The batch was aged 1 hour at room temperature atwhich point HPLC analysis indicated that the reaction was complete. Thereaction mixture was transferred to a 22-L flask and the mixture wasdiluted with water (6.4 L, 8 vol). The two-phase mixture was warmed to50° C. and held at temperature for 16 hours to quench the excess benzylchloroformate. The mixture was transferred hot to a separatory funnel toremove the lower aqueous phase. A rag layer was observed which was takenwith the aqueous layer. The THF layer was filtered through Whatman #1filter paper to remove some particulates, and the mixture wastransferred back to a 22-L flask equipped for distillation. Heptane wasadded in portions and distilled to remove the THF. (Note that it is bestto distill some of the THF out first before adding the first amount ofheptane.) A total of 26.5 L of heptane was added, and the totaldistillate collected was 25 L. At this point, the pot temperature hadreached 97.7° C. and the distillate coming over contained 0.9% THF by ¹HNMR analysis. The mixture was cooled to room temperature and the thickwhite slurry was filtered. The filter cake was washed with heptane (4L). The product was dried in a vacuum oven at 40° C. to give 1257.0 g ofintermediate 5 (92% yield). The HPLC assay was 98.3% (AUC).

Example 4 Preparation of4-(Benzyloxycarbonylamino)-2-fluorophenylboronic acid 6 (R^(1a)═F,R^(1b)═H, R²=Bz, Y═B(OH)₂)

A 22-L, three-neck, round-bottom flask was equipped with an overheadstirrer, temperature probe, 2-L addition funnel, and a nitrogen inletadapter. The flask was charged with intermediate 5 (1.00 kg, 3.08 mol,AMRI lot #CAR-L-18(3)), THF (10 L, 10 vol) and triisopropyl borate(638.2 g, 3.39 mol, 1.1 eq.). The mixture was stirred and cooled to −72°C. in a dry ice/acetone bath. The addition funnel was charged inportions with 2.5 M n-butyllithium (2.59 L, 6.48 mol, 2.1 eq.), whichwas added dropwise to the reaction over approximately 2 hours. Themaximum temperature during the addition was −65° C. The reaction wasdeemed complete by HPLC analysis. The acetone was removed from thecooling bath, and the reaction was quenched with 20% aqueous ammoniumchloride solution (5.5 L), allowing the reaction to warm to −1° C. Thephases were separated and the THF layer was evaporated to dryness. Thecrude product was reslurried in 3:2 ethanol/water (10 L, 10 vol) at roomtemperature for 1 hour. The mixture was filtered and the filter cake wasrinsed with 3:2 ethanol/water (2×2 L). The product was dried in a vacuumoven at room temperature to give 592.8 g of intermediate 6 (66% yield)that was 89.8% (AUC) by HPLC analysis (Method A). The material was muchless pure by ¹⁹F NMR analysis and HPLC analysis at 240 nm (Method C).

Later development of this process used 2.5 volumes of CH₂Cl₂ to reslurrythe crude product in place of 3:2 ethanol/water, which removed thedes-bromo by-product, which was the impurity observed in the ¹⁹F NMRspectrum and the HPLC at 240 nm.

Example 5 Preparation of benzyl(4-(2-(2-methyltetrazol-5-yl)pyridin-5-yl)-3-fluorophenyl)carbamate, 7(Het=2-methyltetrazol-5-yl, R1a=F, R1b=H, R2=Bz)(Ref.: JAS-G-96) (Ref.:CAR-L-93, DUG-AF-202)

To a 5-L, three-neck, round-bottom flask was charged 4 (200.0 g, 0.833mol) followed by 1,4-dioxane (3 L, 15 vol). Crude compound 6 (361.2 g,1.249 mol, 1.5 equiv.), Pd₂(dba)₃ (11.44 g, 0.0125 g, 0.015 equiv.), andPCy₃ (7.0 g, 0.025 mol, 0.03 equiv.) was charged and degassed withnitrogen for 30 minutes. A solution of K₂CO₃ (195.7 g, 1.7 equiv.) inwater (800 mL, 4 vol) was charged, and the reaction was heated to 70° C.The reaction was complete after 1 hour with 0.5 area % of 4 remaining.The reaction was cooled to 50° C., and Darco G-60 (40 g, 0.2 wt) wasadded and stirred for 30 minutes. Celite 545 (40 g, 0.2 wt) was chargedand then the reaction was filtered through Celite 545 (100 g, 0.5 wt)wetted with water (300 mL). The hot filtration into the water from theCelite caused precipitation of the product. Tetrahydrofuran (1.2 L, 6vol) and brine (600 mL, 3 vol) were added, and the product re-dissolvedat room temperature. The phase split was accomplished cleanly (Vmax=28volumes). The dioxane was concentrated and ethanol (1 L, 5 vol) wasadded and concentrated. Then the product was reslurried in ethanol:water(4:1, 2 L, 10 vol) at 70° C., cooled to room temperature over 3 hours,filtered and washed with ethanol (2×400 mL). Compound 7 was isolated in87% yield (292.6 g) with a purity of 97.7% (AUC) by HPLC analysis. The¹H NMR and ¹⁹F NMR indicated the presence of one compound. Pd analysisshowed 135 ppm Pd was in the product.

The intermediate 7 was recrystallized from ethyl acetate to furtherreduce the level of palladium. Intermediate 7 (130 g) and ethyl acetate(3.9 L, 30 volumes) were charged to a 5-L, three-neck, round-bottomflask. The slurry was warmed to 75° C. at which point the solidsdissolved. The hot solution was filtered to remove any palladium black(0.2- to 0.45-μ filters the best) and returned to a clean 5-L flask. Theethyl acetate solution was distilled at atmospheric pressure to remove2.2 L of the ethyl acetate (b.p. 77-78° C.). The solution was cooled to22° C. and the resulting slurry was filtered. The flask and filter cakewere washed with ethyl acetate (3×130 mL) of ethyl acetate. The purifedintermediate 7 was dried in a vacuum oven at 50° C. to give 110.5 g ofintermediate 7 (85% recovery). The HPLC assay of the purifiedintermediate 7 was 98.5% (AUC). The palladium level in the purifiedproduct was 6 ppm. The mother liquor was evaporated to recover 18 g ofcrude product (14% recovery, 2254 ppm Pd).

Example 6 Preparation of(R)-3-(4-(2-(2-methyltetrazol-5-yl)pyridin-5-yl)-3-fluorophenyl)-5-hydroxymethyloxazolidin-2-one, 1 (R═H), also referred to as “TR-700”

A 5-L, three-neck, round-bottom flask was equipped with an overheadstirrer, a thermocouple, a 500-mL addition funnel and a nitrogen-inletadapter. The flask was dried with a heat gun under a flow of nitrogen toan internal temperature of 60° C. The flask was charged withintermediate 7 (110.0 g, 0.272 mol, AMRI lot #DUG-AF-202(1)) andanhydrous THF (2.2 L, 20 vol). The slurry was stirred and a light greensolution formed. The addition funnel was charged with 1.0 M lithiumhexamethyldisilazide (299 mL, 0.286 mol, 1.05 eq.). The LiHMDS solutionwas added dropwise to the solution of intermediate 7 over approximately25 minutes. A red solution formed. The solution was stirred one hour atroom temperature and then DMPU (34.9 g, 0.272 mol, 1 eq) was added, andthe mixture turned to a yellow slurry. The batch was cooled in an icebath to 5.7° C. R-(−)-Glycidyl butyrate (41.25 g, 0.286 mol, 1.05 eq)was then added in one portion. The mixture was stirred in the ice bathfor 0.5 hour and then was warmed to room temperature and stirredovernight. The reaction formed a tan slurry at this point, and HPLCanalysis after 15 hours indicated that there was approximately 87%TR-700, 1.6% intermediate 7, and approximately 7% of the butyrate esterof TR-700. A small amount of sodium methoxide in methanol (11 mL, 0.1vol) was added, and the batch was stirred for 1 hour to remove theresidual ester. The in-process HPLC analysis at this point showed therewas approximately 90.7% TR-700 and 0.2% of the butyrate ester. Thereaction was quenched by the addition of 10% w/w ammonium chloridesolution (1.1 L, 10 vol). A modest exothermic event from 22° C. to 25°C. was observed upon addition of the ammonium chloride solution. Thetwo-phase mixture was distilled to a pot temperature of 70° C.(atmospheric pressure) to remove approximately 2.2 L of the THF. Thisformed a thick slurry which is diluted with water (550 mL, 5 volumes).The slurry was cooled to room temperature (23.6° C.) and was filtered.The filter cake was washed with water (1.1 L, 10 vol) and methanol (550mL, 5 vol) to give TR-700 as a white solid. The wet cake was driedovernight in a vacuum oven at 50° C. to give 89.7 g of TR-700 (89%yield) that was 97.8% (AUC) by HPLC analysis. The TR-700 was furtherpurified by reslurrying in 2.7 L (30 vol) of 4:1 methanol/water at 70°C., cooling to 23° C., filtering and washing with methanol (180 ml).This removed some of the over-alkylated product that is observed. Thepurified TR-700 was recovered in 96% yield (85% overall yield), and thepurity was improved to 98.4% (AUC) by HPLC analysis. The palladiumcontent was 10 ppm.

Example 7 Preparation of(R)-3-(4-(2-(2-methyltetrazol-5-yl)pyridin-5-yl)-3-fluorophenyl)-5-hydroxymethyloxazolidin-2-one dihydrogen phosphate 1 (R═PO(OH)₂) also referred to as“TR-701FA”

A 5-L, jacketed round-bottom flask was equipped with an overhead,mechanical stirrer, addition funnel, thermocouple, nitrogen inlet, and acirculating chiller unit. The flask was charged with TR-700 (70.0 g,0.189 mol), THF (1.4 L, 20 vol), and triethylamine (58.2 g, 0.575 mol, 3eq). The slurry was stirred and the jacket temperature was set to 0° C.The addition funnel was charged with phosphorus oxychloride (87.0 g,0.567 mol, 3 eq) in THF (70 mL, 1 vol). Once the internal temperaturereached 1° C., the POCl₃ solution was added dropwise over 44 minutes.The maximum internal temperature was 2.2° C. The mixture was stirred for3 hours at 1-2° C. at which point HPLC analysis indicated that <0.5% ofthe TR-700 remained. A 5-L, three-neck, round-bottom flask equipped witha Teflon diaphragm pump was charged with water (1.4 L, 20 vol) and wascooled to 3.8° C. in an ice, salt water bath. The reaction mixture waspumped into the quench water subsurface over 1 hour. The maximumtemperature during the quench was 11.9° C. The reactor and pump lineswere rinsed with water (˜210 mL) into the quench vessel. The yellowslurry was stirred overnight. The slurry was filtered through Whatmanpaper, and the filter cake was rinsed with water (700 mL, 10 vol) andmethanol (700 mL, 10 vol). The product was dried at room temperature ina vacuum oven until a constant weight was obtained. The yield of crudeTR-701FA was 81.6 g (96%), and the purity by HPLC analysis (Method B)was 95.3% (AUC).

Example 8 Preparation of(R)-3-(4-(2-(2-methyltetrazol-5-yl)pyridin-5-yl)-3-fluorophenyl)-5-hydroxymethyloxazolidin-2-one phosphate, disodium salt 1 (R═PO₃ 2Na) also referred toas “TR-701”

Crude 1 (R═PO(OH)₂) (60.0 g, 0.133 mol) was charged to a 2-L reactor.Methanol (720 mL, 12 vol) was added and the slurry was stirred at roomtemperature. The 25% sodium methoxide in methanol (86.1 g, 0.398 mol, 3eq) was added dropwise over 13 minutes. The reaction temperatureincreased from 20.4° C. to 26.8° C. during the addition of sodiummethoxide. The slurry was stirred one hour at room temperature and thenwas filtered. The reactor and filter cake were rinsed with methanol (300mL, 5 vol) and acetone (300 mL, 5 vol). The product was dried in avacuum oven at 50-60° C. to give 65.3 g of crude TR-701 (99% yield). Thecrude product was dissolved in water (653 mL, 10 vol) to give a strawcolored solution. The solution was stirred with Darco G-60 carbon (3.3g, 0.05 wt) at room temperature for 30 minutes. The pH of the slurry was7.2, so 5-10 mL of 2 N NaOH was added to the solution to raise the pH to11. The slurry was filtered through Celite 545 (65 g, wetted withwater). Some black color passed through. The filtrate was refilteredthrough a 0.45-μ filter, but some carbon passed through again. Thefiltrate was added dropwise to acetone (2.6 L, 40 vol), and theresulting slurry was stirred overnight for convenience. The slurry wasthen filtered, rinsed with acetone (650 mL), and dried in a vacuum ovenat 50° C. to give 46.9 g of 1 (R═PO₂Na) (71% yield) that was gray incolor. The HPLC purity of this material was 99.0% (AUC), but since itwas gray, it was re-dissolved in water (470 mL). The aqueous solutionwas pH 9.6, so sodium hydroxide solution was added to raise the pH to10. The solution was then filtered through a 0.45-μ filter to removecolor. The filtrate was added dropwise to acetone (1.88 L). The whiteslurry was filtered and was washed with acetone (470 mL). After dryingthe product, the TR-701 weighed 43.2 g (66% overall yield). The HPLCpurity (Method B) was 99.6% (AUC). The other analyses conducted on thislot of 1 (R═PO₂Na) are shown in Table 1.

Example 9 Preparation of PurifiedR)-3-(4-(2-(2-methyltetrazol-5-yl)pyridin-5-yl)-3-fluorophenyl)-5-hydroxymethyloxazolidin-2-one dihydrogen phosphate, 1 (R═PO(OH)₂2

A 3-L, three-neck, round-bottom flask was charged with crude 1(R═PO(OH)₂) (99.8 g, 0.222 mol, AMRI lot #8AK0242C) and water (1 L, 10vol). The pH of this slurry was 2.05. A fresh solution of 1 M sodiumhydroxide solution was prepared by dissolving 50.9% aqueous sodiumhydroxide (39.3 g, 0.50 mol) in a total volume of 0.5 L of water. The 1M sodium hydroxide solution (444 mL, 0.444 mol, 2 eq) was added dropwiseto the free acid slurry. At pH 5.7, the solids dissolved, even thoughonly a little over half the sodium hydroxide solution had been added. Atthe end of the addition the pH was 8.57. Darco G-60 carbon (5.1 g, 0.05wt) was added to the solution and the mixture was stirred for 1 hour atroom temperature. The slurry was filtered through Whatman #1 filterpaper to remove the bulk carbon, and then through a 0.45-μ filter toremove the fines. The straw-colored filtrate was added dropwise to a12-L round-bottom flask containing acetone (4 L, 40 vol). The resultingslurry was stirred for 1 hour at room temperature, was filtered andwashed with acetone (500 mL, 5 vol). The wet cake was loaded into a 3-Lround-bottom flask and was allowed to dry under a nitrogen purgeovernight.

The disodium salt 1 (R═PO₂ 2Na) was re-dissolved in water (1 L, 10 vol)and then was filtered through Whatman #1 filter paper when black fleckswere observed in the solution. The filtrate was diluted with THF (1 L,10 vol). The pH of the aqueous THF solution was 9.57. Freshly prepared 2M hydrochloric acid solution (222 mL, 0.444 mol, 2 eq.) was addeddropwise to pH 1.34. The product did not precipitate until approximately170 mL of the 2 M HCl solution was added. The yellow slurry was filteredand rinsed with water (500 mL, 5 vol) and methanol (500 mL, 5 vol). Thefilter cake cracked as it dried, so it was smoothed out before addingthe rinse solvents. The product was dried in a vacuum oven at 60° C. for19.5 hours to give 79.3 g of 1 (R═P(OH)₂) (80% yield). HPLC analysis(Method B): 99.5% (AUC) t_(R)=5.6 min. ¹H and ³¹P NMR analyses wereconsistent with the assigned structure. The level of residual THF by NMRanalysis was 1600 ppm, and the palladium level was 11 ppm. Sinceextended drying did not remove the THF, future batches were made withuse of ethanol as the antisolvent.

Example 10 Isolation ofbis{[(5R)-3-{3-fluoro-4-[6-(2-methyl-2H-tetrazol-5-yl)pyridin-3-yl]phenyl}-2-oxo-1,3-oxazolidin-5-yl]methyl}dihydrogendiphosphate (the dimer of 1)

Crude 1 from example 8 was dissolved in phosphate buffer andchromatographed on a Gilson preparative HPLC system The mobile phase wasa linear gradient of water and acetonitrile t+0 was 100 5 H2O and T=20was 100% acetonitrile. Fractions were analyzed using analytical HPLC.Those fractions found to be enriched in the Dimer were pooled providinga solution containing over 60% Dimer. Further purification of the Dimerenriched fractions was accomplished on a semi preparative HPLC. Thisyielded pure dimer: accurate mass (m/z 883; calcd. ForC34H31F2N12O11P2=883.1679. found 883.1658, A=2.4 ppm m/z 905 calcd. forC34H30F2N1O11P2Na=905.1498. found 905.1484, A=1.6 ppm) confirming theformula for this compound.

TABLE 1 Analysis of TR-701 (R)-3-(4-(2-(2-methyltetrazol-5-yl)pyridin-5-yl)-3-fruorophenyl)-5-hydroxymethyl oxazolidin-2-one, 1 (R = H) TestResult Appearance White to Off-white ¹H NMR Conforms ³¹P NMR ConformsRetention Time 5.18 min MS m/z 371 HPLC Purity 99.6* HPLC ImpuritiesDimer, 0.09%* Copper Content <1 ppm Palladium Content 1 ppm SodiumContent 8.34% Water Content 5.5% Specific Rotation −34.9° XRPD AmorphousParticle Size 1-300 μm

1. (canceled)
 2. (canceled)
 3. (canceled)
 4. (canceled)
 5. (canceled) 6.(canceled)
 7. (canceled)
 8. (canceled)
 9. (canceled)
 10. (canceled) 11.(canceled)
 12. (canceled)
 13. (canceled)
 14. (canceled)
 15. (canceled)16. (canceled)
 17. (canceled)
 18. (canceled)
 19. (canceled) 20.(canceled)
 21. (canceled)
 22. (canceled)
 23. (canceled)
 24. (canceled)25. (canceled)
 26. (canceled)
 27. (canceled)
 28. (canceled) 29.(canceled)
 30. (canceled)
 31. (canceled)
 32. A compound of the formula:

wherein; R¹a and R¹b are independently selected from H and F, providedthat at least one of R¹a and R¹b is F, R² is selected from the groupconsisting of optionally substituted benzyl and optionally substitutedC₁-C₆ alkyl, and Het is an optionally-substituted five- or six-memberedheterocycle comprising at least one N, O, or S atom.
 33. A compound ofthe formula:

wherein R¹a and R¹b are independently selected from H and F, providedthat at least one of R¹a and R¹b is F, R² is selected from the groupconsisting of optionally substituted benzyl and optionally substitutedC₁-C₆ alkyl, and Y is selected from the group consisting of ZnCl, BF₃,and BR³R⁴, wherein R³ and R⁴ are independently selected from the groupconsisting of OH and optionally-substituted C₁-C₆ mono and dihydricalcohols, and wherein R³ and R⁴ together may form a ring.
 34. (canceled)35. The compound of claim 32 wherein R¹a is F and R¹b is H. 36.(canceled)
 37. (canceled)
 38. The compound of claim 32 wherein Het isoptionally substituted tetrazolyl group.
 39. The compound of claim 38wherein Het is 2-methyl-tetrazol-5-yl.
 40. The compound of claim 32wherein R² is benzyl.
 41. The compound of claim 32, wherein R¹a is F,R¹b is H, R² is benzyl and Het is 2-methyl-tetrazol-5-yl.
 42. Thecompound of claim 33 wherein R¹a is F and R¹b is H.
 43. The compound ofclaim 33 wherein R² is benzyl.
 44. The compound of claim 33 wherein Y isB(OH)₂ or pinacolatoborate.
 45. The compound of claim 33 wherein R¹a isF, R¹b is H, R² is benzyl and Y is B(OH)₂ or pinacolatoborate.
 46. Amethod of purifying the compound of claim 41, comprising providing amixture comprising palladium, the compound and a solvent in which atleast a portion of the compound is dissolved to form a solution; andfiltering the mixture to remove at least a portion of the palladium tothereby obtain a filtrate, the filtrate comprising at least a portion ofthe dissolved compound and a reduced level of palladium as compared tothe mixture.
 47. The method of claim 46, further comprising removing thesolvent from the filtrate.
 48. The method of claim 46, wherein thesolution is a hot solution.